PhD proposal in innovative microscopic imaging at IRIMAS (Institut de Recherche en Informatique, Mathématiques, Automatique et Signal), Université de Haute-Alsace, Mulhouse, France.
High Resolution Tomographic Diffractive Microscopy: Instrumentation and Image processing
Optical microscopy techniques are among the preferred methods for biological studies, thanks to their unique capability of imaging living specimens in 3-D. Tomographic diffractive microscopy (TDM) is a new technique, which permits to image transparent specimens in 3-D , without preparation or staining. It combines micro-holography with tomographic acquisitions, performed by either specimen rotation or illumination scanning. It allows for the measurement of the specimen index of refraction distribution in 3-D, and with a resolution twice better than conventional microscopy (Fig .1).
IRIMAS has built such a microscope [2,3], which has demonstrated its higher lateral resolution in transmission mode [4,5], or improved sectioning capabilities in reflection mode [6,7]. We have also developed a world-premiere system, delivering an isotropic 3-D resolution in the 100 nm range .
Development of the technique, in the framework of the proposed PhD, will concern two domains:
1) instrumental developments: speed of acquisition shall be improved, and new imaging modalities are to be implemented: polarized acquisitions for birefringence measurements, multi-spectral TDM imaging, functionalized tomographic imaging by temporal heterodyning, to detect motions within the sample (such a flowing blood cells for example) etc...
2) image processing: the reconstruction methods we use up-to-know are based on the very fast first Born approximation . They however present limitations. The PhD student will therefore also develop advanced image reconstruction techniques, in order to further improve the resolution (by linear and non-linear deconvolution of the images), or simplify/accelerate acquisitions, by sparse acquisitions combined to elaborate image reconstructions.
Profile: competences in optics and/or image processing and/or computer sciences are necessary.
The selected candidate will join an active and recognized research group. Theoretical and numerical work will be carried out with LaHC St-Etienne and experiences on biological samples will be performed with the Imaging Platform of IGBMC in Strasbourg, as this project is supported by the collaborative project ANR HORUS: High Optical Resolution for Unlabelled Samples.
PhD could start 1st January 2019, when appropriate candidate is identified.
For more information, contact:
Pr. Olivier Haeberlé firstname.lastname@example.org Dr. Nicolas Verrier email@example.com
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(c) GdR 720 ISIS - CNRS - 2011-2018.